FTIR profiling and imaging
FTIR for fast drug discovery: antitumor drugs
We developed a strategy to identify and scoring potential antitumor drugs. Several cancer cell lines are grown in the lab and the impact of anticancer molecules on cell biochemistry is evaluated by infrared spectroscopy. The FTIR spectral signature is used to classify drug mode of action. Innovative modes of action can be singled out quickly.
Phenotyping by infrared spectroscopy
The first study by infrared spectroscopy of tumor cell behavior revealed that the ability of a glioma cell line to kill mice (xenografted) could be precisely predicted from the absorbance of the cell infrared spectrum at only 3 wavenumbers. In the absence of any other marker for the tumor aggressiveness, it became apparent that infrared spectroscopy has a huge potential to help identify specific phenotypes.
This potential was confirmed by studies on pancreatic carcinoma and on parasites (Leishmania sp)
FTIR-imaging of biological and medical samples
Imagine we could directly visualize the difference between a tumour-infiltrating lymphocyte and a normal T-cell and predict if a tumor is of metastatic origin: these are the kind of things FTIR imaging is beginning to perform with more and more success. In our lab, we currently work on mammary tumors an cultured cells. Cells and tissues are visualized using FTIR detection arrays and each pixel contains the local FTIR spectrum. Supervised and unsupervised statistical treatment of the data is critical in this process.
High throughput screening of recombinant protein samples at a microscale
Hundreds of recombinant protein samples screened for conformational stability, folding reproducibility
see the Robotein Plateform (in collaboration with the University of Liège)